Safranine O

CAS: 477-73-6

Ref. 02-B21674

Product Information

• Name: Safranine O
• Synonyms: safranin, 2,8-Dimethylphenosafranine, 3,7-Diamino-2,8-Dimethyl-5-Phenylphenazin-5-Ium Chloride, 3,7-Diamino-2,8-dimethyl-5-phenyl phenazinium chloride, 3,7-Diamino-2,8-dimethyl-5-phenylphenazinium chloride, 3,7-Diamino-2,8-dimethyl-5-phenylphenaziniumchlorid, Basic Pink, Basic Red 2, Brilliant Safranine BR, Brilliant Safranine G, Brilliant Safranine GR, Calcozine Red Y, Chlorure de 3,7-diamino-2,8-dimethyl-5-phenylphenazinium, Cloruro De 3,7-Diamino-2,8-Dimetil-5-Fenilfenazinio, Duasyn Basic Red TH, Gossypimine, Grams safranin solution, Hidaco Safranine, Leather Red HT, Lowacryl Red 2, Lowacryl Red 2 Conc, Mitsui Safranine T, Nippon Kagaku Safranine GK, Nippon Kagaku Safranine T, Phenazinium, 3,7-diamino-2,8-dimethyl-5-phenyl-, chloride, Phenazinium, 3,7-diamino-2,8-dimethyl-5-phenyl-, chloride (1:1), Safranin, Safranin O, Safranin Solution Acc. To Olt, Safranin T, Safranin T solution, Safranin orange, Safranine, Safranine A, Safranine B, Safranine G, Safranine GF, Safranine J, Safranine OK, Safranine OK 70:100, Safranine Superfine G, Safranine T, Safranine TH, Safranine TN, Safranine TS, Safranine Y, Safranine YN, Safranine Zh, SafranineT, Tolusafranine, o-Safranin
• CAS: 477-73-6

Description

It is a redox and adsorption indicator. Employed as a biological stain used in histology, cytology, bacteriology. It is commonly used as a counter stain for gram-negative bacteria in the Gram stain procedure. It has been observed to bind to DNA at a pH 2.21. In the Flemming triple stain procedure it is used to stain for chromosomes. In late Paleozoic pteridosperm cuticles it has shown increased contrast, emphasized cutinization differences, and enhanced the three-dimensional morphology of complex epidermal features when compared with other dyes. The compound has also been used with alcian blue in the histochemical study of the distribution and morphometric characteristics of mast cells. It has also been used to detect mucin and cartilage. This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

• Brand: Alfa Aesar By Thermo Fisher Scientific

Chemical properties

• Molecular weight: 350.85
• Formula: C₂₀H₁₉ClN₄
• Color/Form: Red-brown or blue-green or dark green to black, Powder or crystalline powder
• InChI key: QRYAEWIQIBAZOJ-UHFFFAOYSA-N